The Golgi Stain Service

References:

1. Corsi P: Camillo Golgi??s morphological approach to neuroanatomy. In Masland RL, Portera-Sanchez A and Toffano G (eds.), Neuroplasticity: a new therapeutic tool in the CNS pathology, pp 1-7. Berlin: Springer, 1987.
2. Ramon-Moliner E: The Golgi-Cox technique. In Nauta WJH and Ebbesson SOE (eds.), Contemporary Methods in Neuroanatomy. pp 32-55, New York: Springer, 1970.
3. Graveland GA, Williams RS and DiFiglia M: Evidence for degenerative and regenerative changes in neostriatal spiny neurons in Huntington??s disease. Science 227:770-773, 1985.
4. Robinson TE and Kolb B: Persistent structural modification in nucleus accumbens and prefrontal cortex neurons produced by previous experience with amphetamine. J. Neurosci. 17:8491-8497, 1997.
5. Glaser ME and Van der Loos H: Analysis of thick brain sections by obverse-reverse computer microscopy: application of a new, high clarity Golgi-Nissl stain. J. Neurosci. Methods 4:117-125, 1981.
6. Dahl JP, Wang-Dunlop J, Gonzales C, Goad MEP, Mark RJ and Kwak SP: Characterization of the WAVE1 knock-out mouse: implications for CNS development. J. Neuroscience 23: 3343-3352, 2003.
7. Milatovic D, Zaja-Milatovic S, Montine KS, Horner PJ, and Montine TJ: Pharmacologic suppression of neuronal oxidative damage and dendritic degeneration following direct activation of glial innate immunity in mouse cerebrum. J. Neurochem. 87: 1518-1526, 2003.
   

Products

Rapid GolgiStain Kit   
Price: $630.00  

Golgi-Cox impregnation has been one of the most effective techniques for studying both the normal and abnormal morphology of neurons as well as glia (Ref.1,2). Using Golgi impregnation technique, neuroscientists are able to investigate subtle morphological alterations in dendrites and spines in the brains of animals treated with drugs as well as in the postmortem brains of patients with neurological diseases (Ref.3,4). However, the low yields of stained cells and time-consuming process of the Golgi staining method have been the major obstacles to a variety of its applications to neuropathological assessments.

FD Rapid GolgiStain Kit is designed based on the principle of the methods described by Ramon-Moliner (Ref.2), Glaser and Van der Loos (Ref.5). Not only has this Kit been shown to dramatically optimize and simplify the Golgi impregnation procedure, but it has also been proven as one of the most extremely reliable and sensitive methods for visualizing morphological details of neurons (Ref.6,7).  

One of the advantages of this Rapid GolgiStain Kit is to generate a high yield of Golgi-impregnated cells in multiple regions of the brains, which will allow for morphological assessments of both within- and between structures of the brains.

Terms and Conditions:

• For quality assurance of our service, it is recommended that you discuss with us for preferred perfusion protocol and histology and/or immunolabeling protocols.
• It is suggested that you use Gel-coated microscopic slides for tissue mounting and 0.17um-thick coverslips.
• A 15% of the fee will be due upon authorization of the study, and the remaining fee will be due upon delivery of study results.
• The progress of the service is contingent upon staining quality of tissues, operated by the independent contractor.
• Should early termination occur, Neurodigitech will prorate the cost incurred and invoice the Sponsor. The first portion of the fee is non-refundable.
  

Materials Required, but Not Included:  

• ​Double distilled or deionized water.
• Plastic or glass tubes or vials.
• Histological supplies and equipment, including gelatin-coated microscope slides, coverslips, staining jars, ethanol, xylene or xylene substitutes, resinous mounting medium (e.g. Permount), and a light microscope.
  

Contents:

• Solution A (250 ml)
• Solution B (250 ml)
• Solution C (250 ml x 2)
• Solution D (250 ml)
• Solution E (250 ml)
• Glass Specimen Retriever (2)
• Natural hair paintbrush (3)
• Dropping bottle (1)

Order here!