The Golgi Stain Service

3D neuron model of pyramidal cells
NDT104 Golgi impregnated forebrain
Neurodigitech, a Neuro-CRO biotech, neurohistology, immunohistochemistry and Golgi-Cox Stain Service

The Golgi Stain Service includes:



The unique recipe of our “ready-to-use” Rapid GolgiStain kit has simplified the complex nature of required impregnation procedure and most importantly, it provides an extremely high yield of impregnated neurons across multiple regions of the brain, which expands the extent of assessable ROIs for morphological assessment.Type your paragraph here.

Background

Golgi-Cox impregnation is an effective but capricious method to detect the soma along with entire dendritic arbors and dendritic spines of the neurons. The method was initially developed by Italian physician Camillo Golgi, which then became famously used by Spanish neuroanatomist Santiago Ramón y Cajal to reveal a series of discoveries about the interior architecture of the nervous system.

Since the dendritic arbors occupy about 90-95% of total volume of the neuron, and the dendritic spines are the major sites for synaptic transmission between neurons, the Golgi impregnation thus becomes a useful "neural" tool to examine morphological properties of the nervous system. This significance has been further amplified by the fact that the standard histopathological methods are not able to stain dendrites and/or spines. As a consequence, neural staining methods are unable to detect early changes in neurodegenerative processes involving dendritic atrophy and spine loss and completely ignore neuroplastic changes involving enhanced dendritic branching and/or new functional or aberrant spine formation.

By conducting a longitudinal study (Golgi-impregnation and assessment on various time points of the subjects), the Golgi method is able to detect early and progressive neuronal atrophy and show neuroplasticity and recovery from injury (e.g., re-growth of branching and re-gain of spine density). The Golgi method can also be used to evaluate therapeutic effects of any neuroprotective agents on neural regeneration from the injury.

Digitized confocal stack CA1 pyramidal cells