The Golgi Stain Service
III. 3D modeling of the quantitative neuron:
3D volumetric analysis of mouse hippocampal subfields
*It is noted that our Golgi Stain Service includes 1) In-house Golgi-Cox impregnation (using FD Rapid GolgiStain Kit) and 2) Rapid Golgi impregnation protocols for various types of tissue, such as unfixed, frozen, and paraffin embedded samples. Please consult with our team for evaluation of your protocol and study design.
Golgi Stain Service Instructions:
*We recommend you send us a brief description of your study (see blow), so we can help you generate a reasonable and economical quote for your study! Your email should include:
Terms and Conditions:
IV. Volumetric measurement and estimation of cell population along the anterior-to-posterior axis of the hippocampus:
3D distribution of labeled cells in mouse dentate gyrus
Unbiased stereology has been considered by modern neuroscientists as the preferred method to quantify neuronal morphology of the CNS. This method is especially important for the identification of subtle yet important alterations in the morphology of the CNS based on disturbances during development or in neuropsychiatric and neurodegenerative diseases.
Our Golgi-Cox method is designed for accurate measurements of neuronal elements (including size, shape, and number) obtained in an unbiased way by the implementation of proven software packages and algorithms that include standard geometric overlays, mathematical formulae, counting rules, and systematic random sampling methods. Using this unbiased method, the obtained results will be accurate, efficient and more reliable than other ad hoc quantitative analyses.
With computer-generated systematic random sampling layout and stabilized stage control of spatial (x-y-z) coordinates on every serial section of Region of Interest (ROIs) via Zeiss digital microscopic platform, we are able to quantify properties of 3-dimensional (3D) objects from a stack of 2D serial sections through an object (such as microscopic slides of a solid specimen). Our clients will expect to receive most accurate and reliable morphometric results, namely, a “3D” spatial digital profile of ROIs with a series of digitized data, ready for subsequent digital archiving and data/quality assurance.
I. Quantitative neuron morphology, including dendritic analysis and spine counts/density:
II. Quantitative neuron morphology, including dendritic analysis and spine counts/density: